pierce bca protein assay thermo fisher
The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) total Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Thermo Scientific™ 23227. Pierce BCA Protein Assay Kit. 500 mL Kit. Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
The Thermo Scientific Pierce BCA Assay is always compatible with more detergents, buffers/salts and solvents than any other colorimetric protein assay. Now it's compatible with reducing agents at concentrations routinely used in protein sample buffers!
The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL)...
ItemPierce BCA Protein Assay Reagent A. CompanyThermo Fisher Scientific. ApplicationsProtein Assays and Analysis, Protein Biology, Protein Quantitation. Add to Compare List.
The Thermo Scientific Pierce Detergent Removal Resin enables removal of commonly used ionic, non-ionic and zwitterionic detergents from protein/peptide digest solutions. Protein concentration was determined with the Thermo Scientific Pierce BCA Protein. Assay.
Thermo Scientific™ 23225. Kit de ensayo de proteínas BCA Pierce. Utilizados en más laboratorios que cualquier otro ensayo de proteínas compatible con detergentes, los reactivos BCA Pierce proporcionan una determinación exacta de la concentración de proteínas con la mayoría de los tipos...
Protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and equal amounts of protein were diluted with Laemmli buffer. Samples were heated at 56 °C for 15 min before being separated by SDS-PAGE using Criterion XT Bis-Tris Gels (Bio-Rad, CA).
Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Isolated protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer's protocol. Protein was mixed with 4X NuPAGE LDS Sample Buffer (Thermo Fisher Scientific) containing 50 mM DTT and heated to 70 °C before immunoblotting.
The total protein concentration of the homogenates were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) in the same spectrophotometer and the Enzymatic Browning calculated as the A475nm/mg of total protein. Finally, the Relative Enzymatic Browning was...
A Pierce BCA Protein Assay Kit and N-hydroxysulfosuccinimide (S-NHS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The conventional method using BCA for protein quantification in liquid media was here applied for the determination of hydrazide groups presented on...
The bicinchoninic acid (BCA) assay is available in kit form from Pierce (Rockford, Ill.). This procedure is very applicable to microtiter plate methods. Both a standard assay for concentrated proteins and a micro assay for dilute protein solutions are described below.
Thermo Fisher Scientific. LC5925. Альбумин BCA Protein Assay Reagent A, Pierce, 500 мл. Thermo Fisher Scientific. EIASODC. Набор для анализа люциферазы Pierce Firefly со вспышкой.
Total protein levels were measured with the Pierce BCA protein assay (Thermo Fisher Scientific). Fast protein liquid chromatography was performed on plasma pools, essentially as described previously
Proteins can lose their structure and form polymers because of mutations or changes in their immediate environment which can lead to cell damage and disease. Protein concentrations were determined using Pierce BCA Protein Assay Kit (Thermo Fisher, Waltham, MA).
Bound protein was measured using the Thermo Scientific Pierce 660nm Protein Assay by Binding was calculated using the Thermo Scientific BCA Protein Assay. © 2012 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only.
Pierce BCA Protein Assay or absorption at 360nm. (for vitamin B12). 25. thermoscientific.com/pierce. © 2013 Thermo Fisher Scientific Inc. All rights reserved. These products are supplied for laboratory or manufacturing applications only.
Pierce BCA Protein Assay Kit. 22660. Pierce 660nm Protein Assay. This product ("Product") is warranted to operate or perform substantially in conformance with published For a faxed copy, call 800-874-3723 or contact your local distributor. © 2011 Thermo Fisher Scientific Inc. All rights reserved.
Protein measurement was performed using the Pierce BCA protein assay kit (Thermo Fisher). Digested peptides from each sample were chemically labelled with TMT reagents 10 plex (Thermo Fisher). Peptides were resuspended in a 30 µL resuspension buffer containing 0.1 M TEAB...
Three ready-to-use kits for measuring protein concentration in solutions were used in the study: the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA; 23225), 2-D Quant Kit (GE Healthcare; Little Chalfont, UK 80-6483-56) and Qubit® Protein Assay Kits...
Protein content was measured by Pierce BCA assay (Thermo Fisher Scientific) and normalized between labeled and unlabeled samples. A viability assay was conducted for lung digest samples only. Samples were first resuspended in Live/Dead fixable Aqua stain (Invitrogen) as per...
Nuclear protein expression and gene expression of HIF-2α, a transcription factor for SOD2, were significantly downregulated (more than twofold; P <0.05) with galactose Protein concentration for cell lysates was quantified by using the Pierce BCA protein assay (Thermo Fisher Scientific).
Bicinchoninic (BCA) Assay, as well as Modified Lowry Assay kits were purchased from Thermo Determination of Soluble Protein Concentration. Bicinchoninic Acid (BCA) Assay reagent reacts with 18. Pierce BCA Protein Assay Kit - Thermo Fisher Scientific. [accessed 2017 Apr 10]. https...
BCA Pierce Protein Assay: This assay was conducted to determine the concentration of the extracted proteins. The protein concentration of the crude extracts was assayed by a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Ottawa, Canada) according to the manufacturer's instructions...
The protein concentrations were determined via a Pierce BCA protein assay (Thermo Fisher Scientic). Lysates were incubated for at least 4 h at 4 °C. with anti-FLAG magnetic beads (Thermo Fisher) and washed. according to manufacturer protocol.
Protein concentration was measured using the Pierce® BCA protein assay (Thermo Fisher Scientific). Lysates were pre-cleared using protein G magnetic beads (Cell Signaling Technology, Danvers, MA) for 1 h at 4 °C with rotation. The beads were pelleted via magnetic separation rack and...
in proteins affectingoflPipDidanmd ectoanbtroolliss.mPa(thqwqo. g for 10 min at 4 ◦C and the supernatant was retained. Protein concentration in each sample was determined using a BCA assay (Pierce BCA Protein Assay, Thermo Fisher Scientic).
https://dolpxy.com | pierce bca protein assay thermo fisher