pierce bca protein assay kit thermo 23227

The Thermo Scientific Pierce BCA Protein Assay Kit is a two-component, high-precision, detergent-compatible assay reagent set to measure (A562nm) How the assay works The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly...

Thermo Scientific™ 23227. Pierce BCA Protein Assay Kit. 500 mL Kit. Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research.

Pierce™ BCA Protein Assay Kit. Catalog Numbers 23225 and 23227 Doc. Introduction ™ ™ The Thermo Scientific Pierce BCA Protein Assay Kit is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein.

Thermo Scientific Pierce Protein Assay Technical Handbook Version 2. Table of Contents. Micro BCA Protein Assay Modified Lowry Protein Assay. Pre-Diluted Protein Assay Standards: Kit. Bovine Serum Albumin (BSA) Set. Diluted in 0.9% saline and preserved with.

Details: This BCA Protein Assay Kit is the reducing agent-compatible version of our popular Thermo Scientific Pierce BCA Protein Assay. The kit enables you to measure protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-me thermo scientific 23227.

The Thermo Scientific™ Pierce™ BCA Protein Assay is a detergent-compatible formulation based on bicinchoninic acid (BCA) for the colorimetric detection and quantitation of total protein. This method combines the well-known reduction of Cu+2 to Cu+1 by protein in an alkaline medium...

Protein assay reagents involve either protein-dye binding chemistry (coomassie/Bradford) or protein-copper chelation chemistry. Micro BCA™ Protein Assay Kit. Coomassie Dry Protein Assay Plates from Pierce contain Coomassie dye dried into each well.

Marca: Thermo Scientific™ 23227. 147.25 EUR hasta el 2022-02-28 Use el código promocional "16494" para aplicar la oferta. Utilizados en más laboratorios que cualquier otro ensayo de proteínas compatible con detergentes, los reactivos BCA Pierce proporcionan una determinación exacta de la...

Przedmiot ogłoszenia: Pierce BCA Protein Assay Kit (nr kat.23227, Thermo Scientific). Województwo.

The bicinchoninic acid assay (BCA assay), also known as the Smith assay, after its inventor, Paul K. Smith at the Pierce Chemical Company, now part of Thermo Fisher Scientific, is a biochemical assay for determining the total concentration of protein in a solution (0.5 μg/mL to 1.5 mg/mL)...

The Pierce BCA Protein Assay is one of the most commonly used protein quantitation assays, so we have determined the compatibility of this assay with each Pierce BCA Protein Assay in conjunction with the Compat-Able™ Protein Assay Preparation Reagent Set (Cat.# 23225 or 23227 and Cat.#

Pierce BCA Protein Assay Kit. in 200 µl ultrapure water with 0.01% SDS using a 23G needle and 1 ml syringe, after which samples were quantified for cells by visual particle counter (TC20, BioRad) and for total protein by the Pierce BCA Protein Assay Kit (23227, Thermo Fisher Scientific).

The concentration of supernatant protein was determined by Pierce TM BCA Protein Assay Kit (Thermo, #23227). Next, 20-40 ug denatured protein samples were detected by electrophoresis on 6-12% [depending on the molecular weight of the protein to be tested.

Total protein concentration was determined with Pierce BCA Protein Assay Kit (cat #23227, Thermo Fisher). Equal protein amounts (5-10 µg) were loaded for each cell line. Electrophoresis was performed using NuPage Novex gradient gels (cat #NP0322, Life Technologies)...

Pierce BCA Protein Assay Kit Thermo-Fisher Scientific Cat#23227. Mouse Heme Oxygenase 1 ELISA Kit abcam Cat#ab204524. for 5 min. Then, proteins were separated by electrophoresis using NuPAGE 4%-12% Bis-Tris protein precast gels (Thermo-Fisher.

The Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells is an easy-to-use, comprehensive kit for preparation of clean peptide mixtures from cultured cells for MS analysis. Protein recovery was determined by the Pierce Micro BCA Protein Assay.

All Seahorse microplates were normalized by total protein using the Pierce BCA Protein Assay Kit (Thermo 23227) as per protocol with a BSA protein standard. The BCA assay absorbance was read by a Biotek microplate reader using Gen5 software.

RNA and protein isolation kit were purchased from NucleoSpin (#740933.50, Macherey-Nagel, Dűren, Germany) and Pierce™ BCA Protein Assay Kit were purchased from Thermo (#23227, MA, USA). Anti-GFAP, rabbit polyclonal antibody was purchased from DAKO (#Z0334, CA, USA).

The protein quantitation was performed by Pierce BCA protein assay kit (Thermo, 23227) according to the manufacturer's protocol. Pierce™ Agarose ChIP kit (Thermo, Waltham, MA, USA) was used to identify the enrichment of TFE3 and H3K27ac on the HOXD-AS2 promoter.

The in chemico digestibility assay was also used to assess any apparent dissolved Ag release from the food pellets at the acidic pH of the stomach. Protein was determined in triplicate using 25 μL of sample using a commercially available kit (Pierce BCA protein assay kit 23227, Thermo Fisher).

Набор Pierce™ BCA Protein Assay Kit 1 набор. 23227. Набор Power SYBR™ Green PCR Master Mix 2 x 5 мл. Набор QUBIT DSDNA HS ASSAY KIT, 100 1 KIT набор. Thermo Fisher Scientific. Q3285.

...Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher Scientific GmbH, Waltham, Massachusetts, US) according to the manufacturer's manual. Next, we investigated the expression of proteins of the DNA damage and repair pathways in response to 5-FU treatment in 2D and 3D CRC cultures.

Bicinchoninic acid (BCA) protein assay. To determine total protein content in the final, post-lyophilization or precipitation pellet, a Pierce BCA Protein Assay Kit (Thermo Scientific) was used Absorbance was measured on a Multiskan Spectrum 1500 microplate reader (Thermo Scientific) at a...

2.2.7.12. UCL: BCA The Pierce BCA protein Assay kit (233225, 23227, Thermo Scientific) was also used for some protein concentration assays at UCL for the purposes of the western blot, which used equal protein concentration in each lane.

Perilipin 1 (PLIN1), a crucial lipid droplet protein, can be ubiquitinated and interacts with SQSTM1 directly. Thus, TNFα-induced autophagy is a more Lysates were centrifuged at 13,000 g for 30 min at 4 °C. Protein concentrations of the extracts were determined with a Pierce BCA Protein Assay Kit...

using the Pierce BCA Protein Assay Kit (23227; Thermo Scientic) according to manu-facturer's recommendations. For SDS-PAGE and subsequent western blotting, samples were resuspended in SDS loading dye, heated to 95 C, and sonicated. Samples were sepa-rated either on a NuPAGE...

Pierce BCA Protein Assay Kit (Thermo Scientific) was used for measuring the protein concentration of the samples. This kit provides colorimetric detection and quantification of proteins in a sample. The method is based on the biuret reaction where a color is produced in an alkaline solution when Cu+2 is...

Protein concentration was determined using Pierce BCA Protein Assay Kit (#23227, Thermo Fisher) following the manufacturer's instructions. Immunoblotting was per-formed as described (47). Primary antibodies against ATP13A2 (1:1000), ATP13A3 (1:1000), and GAPDH (1:5000) were incubated...

Cells were scraped from wells, transferred to Eppendorf tubes, and centrifuged at 13,000 rpm for 10 minutes at 4°C. Protein determination of each lysate sample was determined by Pierce BCA Protein Assay Kit (Product no 23227; Thermo Scientific) as per supplier's protocol.

Protein recovery was determined by the Pierce BCA Protein Assay Kit. The Thermo Scientific Pierce Power Blotter is designed specifically for rapid semi-dry transfer of 10-300 kDa proteins from polyacrylamide gels to nitrocellulose or PVDF membranes.

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